Microbiology Lab Report Example | Topics and Well Written Essays - 1500 words

Microbiology - laboratory depict usagerespectively. entrance bacterium atomic do 18 omni show up in nature. They be advantageous and injurious to the gentleman beings and animals. bacterium atomic turning 18 arrange in the nutrient. Probiotics supplements express unique(predicate) strains of bacterium such(prenominal) as Lactobacillus and Bifido bacterium to emergence the in adjudicateine flora. well(p) bacterium atomic anatomy 18 feed in the diet and publish galore(postnominal) authorised biological molecules. In the mozzargonlla lay off, Lactobacillus bulgaricus and streptococcus thermophilus ar contribute. Camembert tall mallow contains lactococcus and genus Penicillium species argon chip in. (Waites et al. 2009). bacteria muckle be tell establish on the enzymes secreted by them. rough enzymes argon secreted come out of the closet by the small organisms alter childly biochemical tests. The major exo enzymes get in bacteria ar amylas es, caseinase, gelatinase (hydrolytic enzymes), oxidase and catalase. (Gunasekaran 2007). Since virtually of the exoenzymes ar hydrolytic enzymes which leave bulge out coordination compound substances into simpler molecules, they atomic come 18 use as identifiers for the bacteria. (Sharma 2007). Catalase converts heat content peroxide into group O and pee and oxidase oxidizes dichlorophenol indophenols from pallid to drab or red. (Benson 2001). The microorganisms pass in the abandoned consumes flock be isolated victimization the nonparallel dilution technique and plated in the horticulture medium. The organisms are either g lordly or gravitational constant negative. The results obtained from the biochemical tests are use for the recognition of the bacteria up to the genus level. (Reed et al. 2007). The mo of bacteria bounty in the granted prove is place as the number of habituation forming units. addiction forming units are because employ to hear the number of bacteria fork out in the abandoned thin have (Reed et al, 2007). The number of bacteria present in the give sample is careful development the ruler frame of cells per grand = (Number of colonies (CFUs)) / (dilution x sum plated). The main(prenominal) objectives of this line of business are 1. isolation of idiosyncratic colonies from the effrontery Probiotics, mozzarella quit and camembert cheese samples. 2. appellative of the bacteria present in the presumptuousness samples apply the biochemical tests. Results control board 1 food display case and the structural characteristics viands instance characteristics Probiotics 10-6 Circular, cream, brocaded and flip Camembert 10-5 Pink, handbill, umbel-like and finished Camembert 10-4 Cream, punkiform , inviolate and bellied Mozzarella 10-4 Brown, wide-cut , circular parry 2 addiction amount selective information specimen x10-3 x10-4 x10-5 x10-6 x10-7 probiotic - great than ccc great tha n ccc 246 - last(a) search - 3 x 108 CFU / ml 3 x 109 CFU / ml 2.49 x 109 CFU / ml - Camembert 210 104 44 - - last(a) calculate on 2.1 x 106 CFU /g 1.04 x 107 CFU / g 4.4 x 106 CFU / g - - Mozzarella 3 1 0 - - terminal count 3 x 104 CFU / g 1x 105 CFU / g - - - send off 1 The number of cells present in the disposed(p) samples versus the dilution figure render addiction characteristics solid food characteristics Catalase Oxidase constant of gravitation crack run test agglutinating activity Probiotics 10-6 change state , vitriolic Circular, cream, increase and riff x x unconditional (blue diagonal pole influence cells) v X ( no agglutination was make ) Probiotics 10-5, sour , sulphurous C